CRISPR/Cas9 is a powerful tool for genome editing.We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose.In our experiments, we targeted two different genes, screened COMPLETE MEGAZYMES for mutations and a
Switzerland is often referred to as a success story for handling its linguistic and cultural diversity.Traditionally License Plate four languages have been spoken in relatively homogeneous territories: German, French, Italian and Rhaeto- Romanic (Romansh).The first three have been national languages since the foundation of the Confederation in 1848
Spinal degenerative joint disease (DJD) is associated with lower pt-insurence back pain (LBP) arising from the degeneration of intervertebral discs (IVD), facet joints, intertransversarii muscles, and interspinous ligaments among other anatomical structures.To circumvent the socioeconomic burdens and often-problematic surgical options imposed by DJ
Food spoilage makes foods undesirable and unacceptable for human use.The preservation of food is essential for human survival, and different techniques were initially used to limit the growth of spoiling microbes, e.g., drying, heating, salting, or fermentation.Water activity, temperature, redox potential, preservatives, and competitive microorgani
Summary: Histone lysine demethylases (KDMs) play critical roles in oncogenesis and therefore may be effective targets for anticancer therapy.Using a time-resolved fluorescence resonance energy transfer demethylation screen assay, in combination with multiple orthogonal validation Rocker Shaft approaches, we identified geldanamycin and its analog 17